ANTIOXIDANT AND ANTICANCER POTENTIAL OF Neolamarckia cadamba (ROXB.) BARK EXTRACT

Current study was conducted to screen the antioxidant and anti cancerous potential of Hydro-methanolic (HM) bark extract of Neolamarckia cadamba.Further presence of various phytochemicals in HM extract of N. cadamba was also accessed by using suitable phytochemical tests.Result of current study suggested the presence of alkaloids, steroids, glycosides, triterpenoids, carbohydrates, flavonoids, tannins and phenols where as the presence of protein and amino acids was not reported in the HM extract of N. cadamba.In vitro antioxidant potential of different doses i.e. 0.025, 0.05, 0.1 and 0.2 mg/ml of N. cadamba HM bark extract were determined using DPPH free radical scavenging method.Results showed significant (p < 0.01) antioxidant efficacy of 0.025, 0.05 and 0.1 mg/ml doses of extract when compared with standard treatment.Evaluation of anticancer activity of different concentrations (10, 20, 40 and 80 µg/ml) of HM extract was done against N1S1 rat hepatoma cancerous cell line using sulforhodamine (SRB) assay.Percentage of control cell growth was -37.66 and -34.13 at 40 µg/ml and 80 µg/ml respectively.Dose dependent decrease in percentage of control cell growth was observed.LC50, TGI and GI50 of HM extract was found to be 75.92,46.73 and 17.46 µg/ml respectively.Present study concludes in vitro antioxidant and anticancerous potential of HM extract of N. cadamba bark.