阿魏酸
香兰素酸
原儿茶酸
丁香酸
木质素
香兰素
化学
麸皮
恶臭假单胞菌
食品科学
香豆酸
生物化学
水解物
对香豆酸
酚酸
有机化学
没食子酸
原材料
抗氧化剂
水解
酶
作者
Priya Upadhyay,Arvind Lali
标识
DOI:10.1080/10826068.2021.1881908
摘要
Biobased chemicals are gaining popularity and market in attempts to mitigate the deteriorating environmental and sustainability issues. Components of renewable agricultural and forest biomass residues are projected to serve as abundant precursors to synthesis of expanding range of products. Agroindustrial wastes comprises of several phenolic compounds associated with lignin via ether linkages such as ferulic acid, p-coumaric, syringic acid and vanillin. These aromatic chemicals have myriad industrial applications. In this study, p-coumaric acid and ferulic acid were found to be two major components in corn bran derived lignin hydrolysate. Engineered Pseudomonas putida KT2440 was constructed and found to convert p-coumaric acid and vanillic acid to protocatechuic acid in >90% and >50% yields, respectively. Engineering the strain included deletion of the gene encoding protocatechuate 3,4-dioxygenase, and overexpression of vanillate-O-demethylase gene from Acinetobacter sp. ADP1.
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