[The effect of aluminum trichloride on expression of phosphorylated tau and Aβ in SH-SY5Y cells].

Objective To investigate the expression of phosphorylated tau (p-tau) and Aβ in SH-SY5Y cells induced by aluminum or/and ApoE e4 allele, and study the interaction between aluminum and ApoE e4 allele. Methods SH-SY5Y cells were assigned to control group, 400 μmol/L AlCl3 group, ApoEe4 transfected group and 400 μmol/L AlCl3 with Apo Ee4 transfected group. The cell viability was measured by CCK-8 assay; the expressions of p-tau and Aβ was determined with ELISA Kit after AlCl3 exposure or or/and ApoE e4 transfection. Results The viability of cells exposed to 400 μmol/L AlCl3 or/and ApoE e4 transfected were significantly lower than that of controls (P<0.05) . The expressions of total tau, tau-181, tau-231, tau-396 and Aβ in 400 μmol/L Al3+ or/and ApoE e4 transfected exposed cells showed significantly higher than those of controls (P<0.05) . Based on the factorial design, a significant interaction exists, and there is a synergistic effect between AlCl3 and ApoE e4 (P<0.05) . Conclusion Aluminum and ApoE e4 allele could increase expression of p-tau and Aβ deposition; there was a synergistic interaction between aluminum and ApoE e4 allele on cell death, tau phosphorylation and Aβ deposition of SH-SY5Y. Key words: Aluminum; Alleles; Neuroblastoma; Gene expression