衰老
脐静脉
端粒
刺
内皮干细胞
细胞生物学
生物
细胞
DNA损伤
生物化学
DNA
体外
航空航天工程
工程类
作者
Chao Xu,Tiantian Cai,Xing-Hai Du
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2023-09-01
卷期号:26
标识
DOI:10.2174/1386207326666230901163717
摘要
Background: Cellular senescence plays a crucial role in age-related diseases. Endothelial senescence is closely associated with age-related vascular disorders. This study aimed to reveal the role of traditional Chinese medicine Liuwei Dihuang (LWDH) in anti-endothelial cell senescence. Methods: Human umbilical vein endothelial cells (HUVECs) were exposed to LPS treatment to induce senescence. Senescence-associated β-galactosidase (SA-β-gal) positive staining, p53 and p16 expression, BrdU staining, and relative telomere length (RTL) experiments were conducted to estimate LPS-induced cellular senescence of HUVECs. Real-time qPCR analysis was performed to identify differentially expressed lncRNAs in LPS-induced senescent HUVECs before and after treatment with LWDH. Bioinformatics analysis and ChIP assay were conducted to explore the mechanism of JPX in the anti-endothelial cell aging effect of LWDH. Results: We first discovered that lncRNA JPX and STING-IRF3 pathways are involved in the process of anti-endothelial senescence of LWDH. Mechanistically, LWDH could reverse abnormally elevated JPX induced by LPS and inhibit the activation of STING, as well as the interaction between JPX and STING. Conclusion: Overall, our study explores the potential therapeutic value of LWDH and provides key insights into the potential avenues for preventing and treating HUVECs senescence.
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