荧光
荧光寿命成像显微镜
双光子激发显微术
材料科学
纳米技术
临床前影像学
生命科学中的荧光
生物医学工程
荧光团
体内
光学
医学
物理
生物
生物技术
标识
DOI:10.1016/j.mtchem.2022.100975
摘要
Bioimaging is increasingly becoming an indispensable tool in disease diagnosis, clinical trials and medical practice. Fluorescence bioimaging is minimally invasive, affordable and portable, with the potential to become a widespread medical imaging technique. Currently, a serious challenge obstructing the large-scale clinical applications of fluorescence technique is the shallow penetration depth. Three-photon fluorescence offers several advantages over near-infrared and two-photon fluorescence, such as deeper penetration, more confined excitation areas and higher resolution. On the other hand, fluorophores displaying solid-state fluorescence are intriguing because they can emit bright fluorescence in the condensed phase, which is beneficial to imaging applications demanding intense emission signals. This review highlights the recent advances in small organic AIEgens for three-photon fluorescence bioimaging in vivo. The progress suggests that three-photon fluorescence imaging offers deep penetration, good photostablity and high signal-to-background contrast, which is valuable in fluorescence imaging in vivo.
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