Stabilized collagen matrix dressing improves wound macrophage function and epithelialization

The FASEB JournalVolume 33, Issue 2 p. 2144-2155 ResearchFree to Read Stabilized collagen matrix dressing improves wound macrophage function and epithelialization Mohamed S. El Masry, Mohamed S. El Masry Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA Department of Plastic and Reconstructive Surgery, Zagazig University, Zagazig, EgyptSearch for more papers by this authorScott Chaffee, Scott Chaffee Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorPiya Das Ghatak, Piya Das Ghatak Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorShomita S. Mathew-Steiner, Shomita S. Mathew-Steiner Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorAmitava Das, Amitava Das Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorNatalia Higuita-Castro, Natalia Higuita-Castro Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorSashwati Roy, Sashwati Roy Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorRaafat A. Anani, Raafat A. Anani Department of Plastic and Reconstructive Surgery, Zagazig University, Zagazig, EgyptSearch for more papers by this authorChandan K. Sen, Corresponding Author Chandan K. Sen cksen@iu.edu Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USACorrespondence: Indiana University School of Medicine, 975 W. Walnut St., Medical Research Library Building, Suite 454, Indianapolis, IN 46202, USA. E-mail: cksen@iu.eduSearch for more papers by this author Mohamed S. El Masry, Mohamed S. El Masry Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USA Department of Plastic and Reconstructive Surgery, Zagazig University, Zagazig, EgyptSearch for more papers by this authorScott Chaffee, Scott Chaffee Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorPiya Das Ghatak, Piya Das Ghatak Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorShomita S. Mathew-Steiner, Shomita S. Mathew-Steiner Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorAmitava Das, Amitava Das Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorNatalia Higuita-Castro, Natalia Higuita-Castro Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorSashwati Roy, Sashwati Roy Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USASearch for more papers by this authorRaafat A. Anani, Raafat A. Anani Department of Plastic and Reconstructive Surgery, Zagazig University, Zagazig, EgyptSearch for more papers by this authorChandan K. Sen, Corresponding Author Chandan K. Sen cksen@iu.edu Department of Surgery, Indiana University Health (IUH) Comprehensive Wound Center, Indiana Center for Regenerative Medicine and Engineering, Indiana University School of Medicine, Indianapolis, Indiana, USA Department of Surgery, The Ohio State University, Wexner Medical Center, Columbus, Ohio, USACorrespondence: Indiana University School of Medicine, 975 W. Walnut St., Medical Research Library Building, Suite 454, Indianapolis, IN 46202, USA. E-mail: cksen@iu.eduSearch for more papers by this author First published: 27 September 2018 https://doi.org/10.1096/fj.201800352RCitations: 2 This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat ABSTRACT Decellularized matrices of biologic tissue have performed well as wound care dressings. Extracellular matrix-based dressings are subject to rapid degradation by excessive protease activity at the wound environment. Stabilized, acellular, equine pericardial collagen matrix (sPCM) wound care dressing is flexible cross-linked proteolytic enzyme degradation resistant. sPCM was structurally characterized utilizing scanning electron and atomic force microscopy. In murine excisional wounds, sPCM was effective in mounting an acute inflammatory response. Postwound inflammation resolved rapidly, as indicated by elevated levels of IL-10, arginase-1, and VEGF, and lowering of IL-lβ and TNF-α. sPCM induced antimicrobial proteins S100A9 and β-defensin-1 in keratinocytes. Adherence of Pseudomonas aeruginosa and Staphylococcus aureus on sPCM pre-exposed to host immune cells in vivo was inhibited. Excisional wounds dressed with sPCM showed complete closure at d 14, while control wounds remained open. sPCM accelerated wound re-epithelialization. sPCM not only accelerated wound closure but also improved the quality of healing by increased collagen deposition and maturation. Thus, sPCM is capable of presenting scaffold functionality during the course of wound healing. In addition to inducing endogenous antimicrobial defense systems, the dressing itself has properties that minimize biofilm formation. It mounts robust inflammation, a process that rapidly resolves, making way for wound healing to advance.—El Masry, M. S., Chaffee, S., Das Ghatak, P., Mathew-Steiner, S. S., Das, A., Higuita-Castro, N., Roy, S., Anani, R. A., Sen, C. K. Stabilized collagen matrix dressing improves wound macrophage function and epithelialization. FASEB J. 33, 2144–2155 (2019). www.fasebj.org Citing Literature Supporting Information Filename Description fsb2fj201800352r-sup-0001.docxapplication/docx, 16.5 KB Supplementary Material 1 fsb2fj201800352r-sup-0002.tifapplication/tif, 8.8 MB Supplementary Material 2 fsb2fj201800352r-sup-0003.tifapplication/tif, 8.7 MB Supplementary Material 3 fsb2fj201800352r-sup-0004.tifapplication/tif, 8.9 MB Supplementary Material 4 fsb2fj201800352r-sup-0005.tifapplication/tif, 8.8 MB Supplementary Material 5 fsb2fj201800352r-sup-0006.tifapplication/tif, 7.4 MB Supplementary Material 6 fsb2fj201800352r-sup-0007.docxapplication/docx, 13.1 KB Supplementary Material 7 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume33, Issue2February 2019Pages 2144-2155 RelatedInformation