胚泡
生物
代谢组
卵母细胞
脂质代谢
新陈代谢
碳水化合物代谢
体外成熟
代谢组学
细胞生物学
转录组
内细胞团
胚胎质量
胚胎
生物化学
胚胎发生
基因表达
生物信息学
基因
作者
Piotr Pawlak,Paulina Lipińska,Ewa Sell‐Kubiak,Arkadiusz Kajdasz,Natalia Derebecka,Ewelina Warzych
标识
DOI:10.1016/j.ydbio.2024.02.004
摘要
Glucose and fatty acids (FA) metabolism disturbances during oocyte in vitro maturation (IVM) affect their metabolism and surrounding cumulus cells, but only inhibition of glucose metabolism decreases embryo culture efficiency. Therefore, the present experiment aimed to reveal if glucose or FA metabolism inhibition leads to the disruption of embryo developmental potential, and to characterize the metabolic landscape of embryos reaching the blastocyst stage. Inhibitors of glucose (IO + DHEA) or FA (ETOMOXIR) metabolism were applied during IVM, and the control group was matured under standard conditions. Blastocysts obtained from experimental and control groups were analyzed with regard to lipidome and metabolome (mass spectrometry), transcriptome (RNA-Seq) and fluorescence lipid droplets staining (BODIPY). We showed that inhibition of glucose and fatty acid metabolism leads to cellular stress response compromising the quality of preimplantation embryos. The inhibition of energy metabolism affects membrane fluidity as well as downregulates fatty acids biosynthesis and gene expression of trophectoderm cell line markers. Therefore, we conclude that oocyte maturation environment exerts a substantial effect on preimplantation development programming at cellular and molecular levels.
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