清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Histone lactylation facilitates hepatocellular carcinoma progression by upregulating endothelial cell‐specific molecule 1 expression

生物 肝细胞癌 癌症研究 组蛋白 细胞生物学 遗传学 基因
作者
Peng Zhao,Chunzhong Qiao,Jiawei Wang,Ye Zhou,Changhe Zhang
出处
期刊:Molecular Carcinogenesis [Wiley]
被引量:1
标识
DOI:10.1002/mc.23794
摘要

Hepatocellular carcinoma (HCC) is a common malignant tumor. Histone lactylation, a novel epigenetic modification, plays a crucial role in various cancers. However, the functional role and underlying mechanism of histone lactylation in HCC progression have not yet been investigated. Histone lactylation levels in HCC tissues and cells were assessed using a densitometric kit and western blot analysis. The role of histone lactylation in cell malignant phenotypes was determined through functional assays in vitro, and a xenograft tumor model was established to verify the function of histone lactylation in vivo. ChIP assay was performed to explore the interaction between histone lactylation and endothelial cell-specific molecule 1 (ESM1). Additionally, gain-and-loss-of-function assays were conducted to investigate the regulatory role of ESM1 in HCC pathogenesis. Histone lactylation levels were increased in HCC tissues and cells, and H3K9 lactylation (H3K9la) and H3K56 lactylation (H3K56la) were identified as the histone modification sites. We observed that H3K9la and H3K56la caused abnormal histone lactylation and were associated with poor prognosis. Functionally, histone lactylation was found to promote HCC cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) process in vitro. However, histone lactylation inhibition with 2-deoxy-d-glucose (2-DG) reduced the malignant phenotypes of HCC cells. In vivo, 2-DG treatment reduced tumor growth and metastasis in the HCC mouse model. Mechanistically, it was revealed that histone lactylation activated ESM1 transcription in HCC cells. ESM1 was expressed at a high level in HCC and exerted a carcinogenic role. Histone lactylation facilitates cell malignant phenotypes, tumor growth, and metastasis by upregulating ESM1 expression in HCC, which reveals the downstream molecular mechanism of histone lactylation and might provide a novel therapeutic target for HCC therapy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
theo完成签到 ,获得积分10
1秒前
16秒前
rafa完成签到 ,获得积分10
17秒前
HCCha完成签到,获得积分10
25秒前
mito应助科研通管家采纳,获得50
26秒前
隐形曼青应助科研通管家采纳,获得10
26秒前
小马哥完成签到,获得积分10
27秒前
doreen完成签到 ,获得积分10
34秒前
珍惜完成签到,获得积分10
38秒前
寒战完成签到 ,获得积分10
39秒前
汉堡包应助一个小胖子采纳,获得10
42秒前
1分钟前
1分钟前
可靠的南霜完成签到 ,获得积分10
1分钟前
阿泽完成签到 ,获得积分10
1分钟前
zuhangzhao完成签到 ,获得积分10
1分钟前
鹰隼游完成签到 ,获得积分10
1分钟前
kier应助一个小胖子采纳,获得10
1分钟前
苗笑卉完成签到,获得积分10
2分钟前
万灵竹完成签到 ,获得积分10
2分钟前
Regina完成签到 ,获得积分10
2分钟前
今后应助一个小胖子采纳,获得10
2分钟前
飞快的冰淇淋完成签到 ,获得积分10
2分钟前
小蘑菇应助科研通管家采纳,获得10
2分钟前
orixero应助一个小胖子采纳,获得10
2分钟前
热心采枫完成签到 ,获得积分10
2分钟前
janer完成签到 ,获得积分10
2分钟前
2分钟前
宇文雨文完成签到 ,获得积分10
2分钟前
2分钟前
共享精神应助一个小胖子采纳,获得10
3分钟前
小西完成签到 ,获得积分10
3分钟前
幸福书琴完成签到 ,获得积分10
3分钟前
SciGPT应助一个小胖子采纳,获得10
3分钟前
Phoenix ZHANG完成签到 ,获得积分10
3分钟前
充电宝应助一个小胖子采纳,获得10
3分钟前
丘比特应助一个小胖子采纳,获得10
3分钟前
lifeup完成签到 ,获得积分10
4分钟前
4分钟前
taster发布了新的文献求助10
4分钟前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Ethnicities: Media, Health, and Coping 700
Genera Insectorum: Mantodea, Fam. Mantidæ, Subfam. Hymenopodinæ (Classic Reprint) 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3088549
求助须知:如何正确求助?哪些是违规求助? 2740715
关于积分的说明 7561203
捐赠科研通 2390734
什么是DOI,文献DOI怎么找? 1267982
科研通“疑难数据库(出版商)”最低求助积分说明 613947
版权声明 598678