维斯坎
细胞生物学
化学
细胞外基质
细胞凋亡
纤维连接蛋白
氧化应激
整合素
细胞
蛋白多糖
生物化学
生物
作者
Yaojiong Wu,Jin Wu,Daniel Y. Lee,Albert Yee,Liu Cao,Yaou Zhang,Chris Kiani,Burton B. Yang
标识
DOI:10.1016/j.matbio.2004.11.007
摘要
Oxidant injury plays a critical role in the degenerative changes that are characterized by a decline in parenchymal cell numbers and viability, and occur with aging and in the etiology of many diseases. The extracellular proteoglycan versican is widely distributed in the extracellular matrix surrounding the cells. This study examines whether versican plays a role in protecting cells from free radical-induced apoptosis. Stable expression of versican or its C-terminal domain significantly decreased H(2)O(2)-induced cellular apoptosis. Cells in adherent monolayer were more resistant to H(2)O(2)-induced apoptosis than cells cultured in suspension. While vigorous trypsinization caused integrin cleavage and rendered the cells more susceptible to H(2)O(2)-induced damages, expression of versican or its C-terminal domain enhanced cell attachment and expression of beta1 integrin and fibronectin. Enhanced cell-matrix interaction by addition of manganese (MnCl(2)) to cultures also significantly diminished H(2)O(2)-induced apoptosis. The results suggest that versican plays an important role in reducing oxidant injury through an enhancement of cell-matrix interaction.
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