化学
阿魏酸
紫外线
活性氧
部分
激进的
阳离子聚合
大肠杆菌
流式细胞术
衍生工具(金融)
辐照
光化学
抗氧化剂
核化学
立体化学
生物化学
有机化学
经济
核物理学
物理
金融经济学
基因
遗传学
生物
量子力学
作者
Atsushi SHIRAI,Masato Kajiura,Takeshi Ōmasa
摘要
Abstract Ultraviolet‐ A ( UV ‐ A )‐mediated bactericidal activity was enhanced by combined treatment with trans ‐ferulic acid ( trans ‐ FA , compound 1 ) or its derivatives. Derivative compounds 4 and 10 contain a phenyl group or an l ‐tyrosine HC l tert ‐butyl ester, respectively, linked to the carboxyl group of trans ‐ FA . Of the three compounds, 10 exhibited the highest synergistic activity in a photobactericidal assay based on treating Escherichia coli with a derivative compound and UV ‐ A irradiation (wavelength 350–385 nm). Inactivation of viable cells at a 4.9 J cm −2 UV ‐ A fluence increased from 1.90 to 5.19 logs in the presence of 10 (100 μ m ); a 4.95‐log inactivation was achieved with 10 (5 μ m ) and a 7.4 J cm −2 UV ‐ A fluence. Addition of antioxidants significantly suppressed photosynergistic bactericidal activity, suggesting that reactive oxygen species ( ROS ) are involved in the combined bactericidal mechanism. Flow cytometry revealed that combined treatment with UV ‐ A and compound 10 , which showed the highest photobactericidal activity, generates an excess of oxidative radicals in bacterial cells. The bactericidal activity of compound 10 may be due to electrostatic interaction between the molecule's cationic moiety and the cell surface, followed by amplification of ROS generation in the cells.
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