基因敲除
癌症研究
乳腺癌
癌变
细胞生长
转移
生物
癌症
转录因子
三阴性乳腺癌
细胞培养
医学
内科学
基因
遗传学
作者
Jin-Yan Liu,Yan-Nan Jiang,Hai Huang,Jin-Fu Xu,Ying-Hui Wu,Qiang Wang,Yue Zhu,Bo Zheng,Cong Shen,Wei-Feng Qian,Jun Shen
摘要
Breast cancer is among the most common malignant cancers in women. B-cell-specific Moloney murine leukemia virus integration site 1 (BMI-1) is a transcriptional repressor that has been shown to be involved in tumorigenesis, the cell cycle, and stem cell maintenance. In our study, increased expression of BMI-1 was found in both human triple negative breast cancer and luminal A-type breast cancer tissues compared with adjacent tissues. We also found that knockdown of BMI-1 significantly suppressed cell proliferation and migration in vitro and vivo. Further mechanistic research demonstrated that BMI-1 directly bound to the promoter region of CDKN2D/BRCA1 and inhibited its transcription in MCF-7/MDA-MB-231. More importantly, we discovered that knockdown of CDKN2D/BRCA1 could promote cell proliferation and migration after repression by PTC-209. Our results reveal that BMI-1 transcriptionally suppressed BRCA1 in TNBC cell lines, whereas in luminal A cell lines, CDKN2D was the target gene. This provides a reference for the precise treatment of different types of breast cancer in clinical practice.
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