吉西他滨
吖啶橙
肺癌
癌细胞
化学
癌症研究
动态光散射
细胞毒性
聚乙二醇
材料科学
癌症
细胞凋亡
核化学
体外
纳米技术
纳米颗粒
医学
肿瘤科
生物化学
内科学
标识
DOI:10.1088/2053-1591/ac9118
摘要
Abstract Lung carcinoma (LC) is rare cancer in most parts of the world but is common cancer in southern Asia. Local recurrent disease and distant metastasis of LC are still mysterious challenges. This investigation was to effectively fabricate and evaluate the therapeutic efficiency of Gemcitabine (GEM) and Losmapimod (LOS) encapsulated into polyethylene glycol (PEG)-polylactic-co-glycolic acid (PLGA)-NPs (GEM/LOS@NPs), which exhibited inhibition of in vitro lung cells proliferation. GEM/LOS@NPs with a diameter of ∼100 nm have been constructed employing an enhanced double-emulsion (W/O/W) method. The GEM/LOS@NPs’ particle size was studied through transmission electron microscopic (TEM) techniques and dynamic light scattering (DLS) measurement. After successfully fabricating GEM/LOS@NPs, the anticancer ability of A549 and H460 lung cancer cells was investigated. Biochemical assays such as AO/EB (acridine bromide/orange ethidium) and nuclear staining investigated the cancer cell’s morphology and death. The cell death of the cancer cells was synergically examined by ROS analysis. Overall, these results suggest that highly toxic drugs can be rationally transformed into self-deliverable and pharmacologically efficient monotherapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI