Unraveling Dynamic Transcriptomic Changes in Sheep's Lactating Mammary Gland Following Escherichia coli Lipopolysaccharide Exposure

大肠杆菌 乳腺 脂多糖 生物 转录组 哺乳期 微生物学 内分泌学 怀孕 生物化学 遗传学 基因表达 基因 癌症 乳腺癌
作者
R. Pelayo,Beatriz Gutiérrez‐Gil,H. Marina,Pablo A. S. Fonseca,María Alonso-García,J. J. Arranz,Aroa Suárez‐Vega
出处
期刊:Journal of Dairy Science [Elsevier BV]
标识
DOI:10.3168/jds.2024-25009
摘要

Mammary gland infections constitute a significant challenge in dairy sheep, impacting productivity and welfare. Temporal RNA-Seq provide a valuable approach to evaluate the evolution of the host defensive molecular mechanisms triggered by mastitis caused by external agents or events. This study aimed to characterize the transcriptomic response of sheep mammary glands to an intramammary inflammation induced with an Escherichia coli lipopolysaccharide (LPS) inoculation based on RNA-Seq samples generated from milk somatic cells collected at 3 time points: pre-inoculation (0 h), and 6 h and 24 h post-LPS inoculation. The differential expression analyses between the analyzed time points were performed using 2 statistical approaches: one parametric (DESeq2) and one non-parametric (Wilcoxon rank sum test). The differentially expressed genes (DEGs) commonly identified by both approaches encompass 5,872 for the 0 h versus 6 h comparison, 4,063 for the 0 h versus 24 h comparison, and 1,034 for the 6 h versus 24 h comparison. At both 6 h and 24 h, transcriptomic data highlighted a significant decrease in the expression of genes linked to metabolic processes crucial for milk protein and lipid synthesis within the mammary gland. Concurrently, increased expression of genes related to the neutrophil attraction was observed for 6 and 24 h, with differences in gene expression between DEGs with the highest expression at 6 h, related to T cell activation, type I interferon-mediated signaling pathway, and 24 h, related to cell-cell neutrophil adhesion extravasation or epithelial cell proliferation. In summary, this study reveals how the sheep mammary gland transcriptome responds dynamically to an LPS inoculation, providing a comprehensive understanding of how gene expression patterns evolve over time and shedding light on the molecular mechanisms driving the initial defensive response of the mammary gland against potential inflammatory challenges.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
我是老大应助KEHUGE采纳,获得10
刚刚
喜之郎发布了新的文献求助10
刚刚
song完成签到,获得积分10
1秒前
2秒前
书宁关注了科研通微信公众号
2秒前
烟花应助犹豫的依波采纳,获得10
3秒前
烟花应助小蜜蜂采纳,获得10
3秒前
福尔摩琪完成签到,获得积分10
3秒前
Starry发布了新的文献求助10
4秒前
酷酷的皮皮虾完成签到,获得积分10
4秒前
山鬼发布了新的文献求助10
4秒前
fuhao发布了新的文献求助30
4秒前
www完成签到 ,获得积分10
5秒前
CHEN.CHENG发布了新的文献求助20
5秒前
陶子完成签到,获得积分10
6秒前
6秒前
AXEDW完成签到,获得积分10
6秒前
daisy发布了新的文献求助10
7秒前
Badass完成签到,获得积分10
7秒前
KST发布了新的文献求助10
8秒前
sci喷涌而出完成签到,获得积分20
8秒前
8秒前
zxzx完成签到,获得积分10
8秒前
汉堡包应助ddd采纳,获得10
9秒前
9秒前
五个跳舞的人完成签到,获得积分10
9秒前
10秒前
懒羊羊完成签到,获得积分10
11秒前
11秒前
苏苏完成签到,获得积分10
11秒前
呆萌代桃发布了新的文献求助10
11秒前
dfgh完成签到,获得积分10
11秒前
飞云发布了新的文献求助10
11秒前
Aoia完成签到,获得积分10
11秒前
无昵称发布了新的文献求助10
12秒前
一一发布了新的文献求助10
13秒前
勾股定理发布了新的文献求助10
13秒前
SRJ完成签到,获得积分20
15秒前
15秒前
子衿发布了新的文献求助10
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969322
求助须知:如何正确求助?哪些是违规求助? 3514152
关于积分的说明 11172188
捐赠科研通 3249407
什么是DOI,文献DOI怎么找? 1794832
邀请新用户注册赠送积分活动 875437
科研通“疑难数据库(出版商)”最低求助积分说明 804781