Effects of isosakuranetin on cerebral infarction and blood brain barrier damage from cerebral ischemia/reperfusion injury in a rat model

医学 半影 缺血 脑梗塞 梗塞 麻醉 H&E染色 血脑屏障 大脑中动脉 再灌注损伤 脑损伤 染色 病理 内科学 中枢神经系统 心肌梗塞
作者
Adchara Janyou,Aida Moohammadaree,Pichaya Jumnongprakhon,Chainarong Tocharus,Ratchanaporn Chokchaisiri,Apichart Suksamrarn,Jiraporn Tocharus
出处
期刊:Journal of Biomolecular Structure & Dynamics [Informa]
卷期号:42 (2): 1064-1071 被引量:2
标识
DOI:10.1080/07391102.2023.2205940
摘要

This study investigated the effects of isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) on cerebral infarction and blood brain barrier (BBB) damage in cerebral ischemia and reperfusion (I/R) in a rat model. The right middle cerebral artery was occluded for 2 h followed by reperfusion. The experimental rats were divided into five groups: a sham, or control group; vehicle group; and 5 mg/kg, 10 mg/kg, and 20 mg/kg bodyweight isosakuranetin-treated I/R groups. After 24 h of reperfusion, the rats were tested using a six-point neurological function score. The percentage of cerebral infarction was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) staining. BBB leakage was determined by Evan Blue injection assay and brain morphology changes were observed under light microscopy following staining with hematoxylin and eosin (H&E). The results of neurological function score revealed that isosakuranetin reduced the severity of neurological damage. A dose of 10 and 20 mg/kg bodyweight of isosakuranetin significantly decreased the infarct volume. All three doses of isosakuranetin significantly decreased Evan Blue leakage. The penumbra area of the I/R brains revealed the characteristics of apoptotic cell death. Therefore, isosakuranetin-treated I/R attenuated the brain damage from cerebral I/R injury and further investigation of the mechanisms warrant further investigation to assist in the development of protective strategies against cerebral I/R injury in clinical trials.Communicated by Ramaswamy H. Sarma
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
刚刚
千早爱音完成签到 ,获得积分10
刚刚
Ava应助ciooli采纳,获得30
刚刚
leeleetyo发布了新的文献求助10
1秒前
1秒前
快不了完成签到,获得积分10
2秒前
游一完成签到,获得积分10
2秒前
3秒前
AHA完成签到,获得积分10
3秒前
酷波er应助不可思宇采纳,获得10
3秒前
滴滴滴完成签到 ,获得积分10
5秒前
5秒前
loong发布了新的文献求助10
5秒前
生科爱好者完成签到,获得积分10
6秒前
STZHEN完成签到,获得积分10
6秒前
6秒前
陶醉雨兰发布了新的文献求助30
7秒前
hope应助infinite采纳,获得10
7秒前
8秒前
10秒前
10秒前
10秒前
Sunflower完成签到 ,获得积分10
11秒前
炙热小馒头完成签到,获得积分10
12秒前
Sun完成签到,获得积分10
12秒前
传奇3应助loong采纳,获得10
12秒前
dxwy应助loong采纳,获得10
12秒前
12秒前
RRR完成签到,获得积分10
13秒前
高兴冬灵完成签到,获得积分10
13秒前
Lucas应助孙永胜采纳,获得10
13秒前
13秒前
所所应助甜甜映菡采纳,获得10
14秒前
ciooli发布了新的文献求助30
15秒前
15秒前
peace and love完成签到,获得积分10
16秒前
16秒前
17秒前
可爱的人雄关注了科研通微信公众号
17秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3148222
求助须知:如何正确求助?哪些是违规求助? 2799394
关于积分的说明 7834549
捐赠科研通 2456604
什么是DOI,文献DOI怎么找? 1307321
科研通“疑难数据库(出版商)”最低求助积分说明 628124
版权声明 601655