脚手架
表面改性
生物相容性
材料科学
极限抗拉强度
化学工程
表面粗糙度
骨组织
复合材料
生物医学工程
医学
工程类
冶金
作者
Jie Meng,Francesco Boschetto,Shinichi Yagi,Elia Marin,Tetsuya Adachi,Xuefei Chen,Giuseppe Pezzotti,Shinichi Sakurai,Sono Sasaki,Takashi Aoki,Hideki Yamane,Huaizhong Xu
出处
期刊:Biomaterials advances
日期:2022-01-31
卷期号:135: 112686-112686
被引量:27
标识
DOI:10.1016/j.msec.2022.112686
摘要
As an emerging additive manufacturing (AM) technique, melt electrospinning writing (MEW) is used to fabricate three-dimensional (3D) submicron filament-based scaffolds with adjustable pore size and customized structure for bone regeneration. Poly(L-lactic acid) (PLLA) scaffold with excellent biodegradability and biocompatibility is first successfully manufactured using our self-assembled MEW device. However, the ultralow cell affinity and poor bioactivity severely hamper their practical applications in bone tissue engineering. These issues are caused by the severe inherent biologically inert, hydrophobicity as well as the smooth surface of the MEW PLLA filaments. In this study, a green and robust alkaline method is applied to modify the scaffold surface and to improve the bioactivity of the MEW PLLA scaffold. Without deterioration in mechanical property but robust surface hydrophilicity, the optimal MEW PLLA scaffold shows promoted surface roughness, enhanced filament tensile modulus (~ 2 folds of the as-prepared sample), and boosted crystallizability (ultrahigh WAXD intensity). Moreover, after being cultured with KUSA-A1 cells, the 0.5 M NaOH, 2 h treated MEW PLLA scaffold exhibits higher osteoinductive ability and increased immature bone tissue amounts (3 times of controlled scaffold). Thus, the flexible surface functionalization by the specific alkaline treatment was found to be an effective method for the preparation of bioactivated MEW PLLA scaffolds with promoted bone regeneration.
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