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Haploid androgenetic development of bovine embryos reveals imbalanced WNT signaling and impaired cell fate differentiation

生物 Wnt信号通路 SOX2 同源盒蛋白纳米 胚泡 胚胎 转录组 胚胎发生 遗传学 囊胚 细胞生物学 胚胎干细胞 男科 信号转导 基因 基因表达 诱导多能干细胞 原肠化 医学
作者
Luis Águila,Ricardo Perecin Nociti,Rafael Vilar Sampaio,Jacinthe Therrien,Flávio Vieira Meirelles,Ricardo Felmer,Lawrence C. Smith
出处
期刊:Biology of Reproduction [Oxford University Press]
卷期号:109 (6): 821-838 被引量:2
标识
DOI:10.1093/biolre/ioad124
摘要

Abstract Haploid embryos have contributed significantly to our understanding of the role of parental genomes in development and can be applied to important biotechnology for human and animal species. However, development to the blastocyst stage is severely hindered in bovine haploid androgenetic embryos (hAE). To further our understanding of such developmental arrest, we performed a comprehensive comparison of the transcriptomic profile of morula-stage embryos, which were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of transcripts associated with differentiation in haploid and biparental embryos. Among numerous disturbances, results showed that pluripotency pathways, especially the wingless-related integration site (WNT) signaling, were particularly unbalanced in hAE. Moreover, transcript levels of KLF4, NANOG, POU5F1, SOX2, CDX2, CTNNBL1, AXIN2, and GSK3B were noticeably altered in hAE, suggesting disturbance of pluripotency and canonical WNT pathways. To evaluate the role of WNT on hAE competence, we exposed early Day-5 morula stage embryos to the GSK3B inhibitor CHIR99021. Although no alterations were observed in pluripotency and WNT-related transcripts, exposure to CHIR99021 improved their ability to reach the blastocysts stage, confirming the importance of the WNT pathway in the developmental outcome of bovine hAE.

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