味精
鲜味
乙酰胆碱
内分泌学
受体
内科学
化学
河豚毒素
生物
生物化学
医学
品味
作者
Molly S. Crowe,Hongxia Wang,Bryan A. Blakeney,Sunila Mahavadi,Kulpreet Singh,Karnam S. Murthy,John R. Grider
摘要
Abstract Background l ‐amino acids, such as monosodium glutamate (MSG), activate the umami receptor T1R1/T1R3. We previously showed increased peristalsis in response to activation of T1R1/T1R3 by MSG in mouse colon. However, the expression and function of these receptors in the different regions of the stomach are not clear. Methods Mouse gastric smooth muscle cells (SMCs) were isolated and cultured in Dulbecco’s Modified Eagle Medium. Expression of T1R1 and T1R3 was measured by RT‐PCR and Western blot. The effect of MSG with and without inosine monophosphate (IMP, an allosteric activator of T1R1/T1R3) on acetylcholine (ACh)‐induced contraction was measured in muscle strips and isolated SMCs by scanning micrometry. The effect of MSG with or without IMP on activation of G proteins and ACh‐induced Ca 2+ release was measured in SMCs. Key Results Monosodium glutamate inhibited ACh‐induced contractions in muscle strips from both antrum and fundus and the effect of MSG was augmented by IMP; the effects were concentration‐dependent and not affected by the nitric oxide synthase inhibitor, L‐NNA, or tetrodotoxin suggesting a direct effect on SMCs. In isolated gastric SMCs, T1R1 and T1R3 transcripts and protein were identified. Addition of MSG with or without IMP inhibited ACh‐induced Ca 2+ release and muscle contraction; the effect on contraction was blocked by pertussis toxin suggesting activation of Gα i proteins. MSG in the presence of IMP selectively activated Gα i2 . Conclusions and Inferences Umami receptors (T1R1/T1R3) are present on SMCs of the stomach, and activation of these receptors induces muscle relaxation by decreasing [Ca 2+ ] i via Gα i2 .
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