Profiles of novel high-molecular-weight synthetic antioxidants in urine and associated child exposure in China

The aim of this study is to investigate the exposure of novel high-molecular-weight (HMW) synthetic antioxidants (AOs), including nine synthetic phenolic antioxidants (SPAs), one low-molecular-weight (LMW) SPA, two organophosphite antioxidants (OPAs) as well as one transformation product in children's urine from eastern (n = 82) and western (n = 105) China. For the first time, all analytes were detected in children's urine such as the representative HMW SPAs pentaerythritol tetrakis(3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate) (AO1010, median = 0.447 ng/mL), octadecyl-3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate (AO1076, median = 0.0300 ng/mL), and 1,3,5-tris[(3,5-di-tert-butyl-4-hydroxyphenyl)methyl]-1,3,5-triazinane-2,4,6-trione(1,2-dioxoethylene)bis(iminoethylene) (AO3114, median = 0.0166 ng/mL) and representative OPAs bis(2,4-di-tert-butylphenyl) pentaerythritol diphosphite (AO626, median = 0.00216 ng/mL), tris(2,4-di-tert-butylphenyl) phosphite (AO168, median = 0.0296 ng/mL) as well as its transformation product tris(2,4-di-tert-butylphenyl) phosphate (AO168O, median = 1.53 ng/mL). Significant differences were observed in the concentrations of AO1010, AO3114, AO168, and AO168O between urine samples from eastern and western China (p < 0.01). The high-frequency combination of AOs from binary to a mixture of six AOs was acquired, which would provide a better investigation of the mixture toxicity. The high estimated daily intakes of AO1010 (85.4 ng/kg/day), AO1076 (10.2 ng/kg/day), AO3114 (4.50 ng/kg/day), and AO168 (1231 ng/kg/day) were less than the values of the tolerable daily intake (3,020,000, 1,500,000, 10,000,000, and 580,000 ng/kg/day for AO1010, AO1076, AO3114, and AO168, respectively), indicating low health risk to children. Our findings showed the co-occurrence of those novel AOs and transformation products in children, the overall risks associated with the mixture of transformation products and the mixture with other emerging pollutants need to be considered when assessing the risks of AOs in further studies.