Liquid chromatography of pyridine nucleotides and associated compounds and isolation of several analogs of nicotinamide adenine dinucleotide phosphate

Abstract A single-column technique is described for separating the naturally occurring nicotinic acid- and nicotinamide-containing nucleosides and nucleotides. A unique feature of the system is the use of conductivity measurements as a reproducible means of peak identification. The chromatographic system has been used to isolate a number of NADP + analogs and to characterize their enzymatic hydrolysis products. These analogs include the α-anomer of NADP + , the nicotinic acid analog of NADP + , and a series of NADP + derivatives that contain phosphate in the 3′-position.