Enucleation of mammalian cells with cytochalasin B

Abstract L cells and chinese hamster cells growing on coverslips can be enucleated by centrifugation at 3 000 g (glass coverslips) or 5 800 g (plastic coverslips) for 40 min at 35 ° to 37 °C in medium containing 10 μg/ml of cytochalasin B. The enucleated cells incorporate 3H-leucine, support replication of vaccinia virus DNA, and are viable for at least 3 days.