Bio-inspired self-pumping microfluidic device for cleaning of urea using reduced graphene oxide (rGO) modified polymeric nanohybrid membrane

In vitro technology facilitates the replication of in vivo tissues more accurately than conventional cell-based artificial organs, enabling researchers to mimic both the structural and functional characteristics of natural systems. Here, we demonstrate a novel spiral-shaped self-pumping microfluidic device for the cleaning of urea by incorporating reduced graphene oxide (rGO) modified a Polyethersulfone (PES) nanohybrid membrane for efficient filtration capacity. The spiral-shaped microfluidic chip is a two-layer configuration of polymethyl methacrylate (PMMA) integrated with the modified filtration membrane. In essence, the device replicates the main features of the kidney (Glomerulus), i.e., a nano-porous membrane modified with reduced graphene oxide to separate the sample fluid from the upper layer and collect the biomolecule-free fluid through the bottom of the device. We have achieved a cleaning efficiency of 97.94 ± 0.6 % using this spiral shaped microfluidic system. The spiral-shaped microfluidic device integrated with nanohybrid membrane has potential for organ-on-a-chips applications.