已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Effects of photodynamic therapy in the normal mouse tongue

医学 血卟啉 溴脱氧尿苷 舌头 光动力疗法 养生 水肿 病理 免疫组织化学 男科 核医学 外科 化学 有机化学
作者
B. Michael,Kazuo Sano,Tsugio Inokuchi
出处
期刊:Journal of Oral and Maxillofacial Surgery [Elsevier BV]
卷期号:51 (10): 1129-1134 被引量:11
标识
DOI:10.1016/s0278-2391(10)80452-5
摘要

The effects of photodynamic therapy (PDT) on the normal mouse tongue were investigated. After using various doses (2.5, 10, and 20 mg/kg body wt) of the photosensitizing drug hematoporphyrin oligomers and 90 or 180 J/cm2 red light (630-nm) emitted from a pulsed neodymium:yttrium-aluminum-garnet dye laser was used to activate it 3 or 24 hours later. It was found that the 20-mg/kg dose elicited a severe response that included extensive vesicular and edema formation. A less severe response was observed with 10 mg/kg of the drug and low-power light (5 mJ/cm2/pulse) periodically delivered (1 hour interval between two 30-minute photoradiations). Such a regimen, however, produced more damage when compared with the higher power (15 mJ/cm2), continuous light delivery counterpart. Healing, except for the protocol with only a 3-hour drug-light interval, was attained by 5 days post-PDT as indicated by regeneration with histologically normal tissues and quantitatively a return to untreated, control values for cross-sectional areas and number of blood vessels. Bromodeoxyuridine immunohistochemistry disclosed an immediate increase in the labeling indices, ie, the percentage of S phase cycling cells, indicating stimulated cell proliferation secondary to repair and fast repopulation of the epithelium. Under the commonly used protocols, PDT was provided safely to the mouse tongue. The regimen of low drug dose and low power of light periodically delivered appears to be the most acceptable method. These parameter-dependent results may partly form the basis for the judicious application of PDT to the oral cavity. The effects of photodynamic therapy (PDT) on the normal mouse tongue were investigated. After using various doses (2.5, 10, and 20 mg/kg body wt) of the photosensitizing drug hematoporphyrin oligomers and 90 or 180 J/cm2 red light (630-nm) emitted from a pulsed neodymium:yttrium-aluminum-garnet dye laser was used to activate it 3 or 24 hours later. It was found that the 20-mg/kg dose elicited a severe response that included extensive vesicular and edema formation. A less severe response was observed with 10 mg/kg of the drug and low-power light (5 mJ/cm2/pulse) periodically delivered (1 hour interval between two 30-minute photoradiations). Such a regimen, however, produced more damage when compared with the higher power (15 mJ/cm2), continuous light delivery counterpart. Healing, except for the protocol with only a 3-hour drug-light interval, was attained by 5 days post-PDT as indicated by regeneration with histologically normal tissues and quantitatively a return to untreated, control values for cross-sectional areas and number of blood vessels. Bromodeoxyuridine immunohistochemistry disclosed an immediate increase in the labeling indices, ie, the percentage of S phase cycling cells, indicating stimulated cell proliferation secondary to repair and fast repopulation of the epithelium. Under the commonly used protocols, PDT was provided safely to the mouse tongue. The regimen of low drug dose and low power of light periodically delivered appears to be the most acceptable method. These parameter-dependent results may partly form the basis for the judicious application of PDT to the oral cavity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Linbo发布了新的文献求助10
1秒前
Tianji完成签到,获得积分20
2秒前
发粪涂墙发布了新的文献求助10
3秒前
keplek完成签到 ,获得积分10
5秒前
6秒前
阿烨完成签到,获得积分10
7秒前
一只半夏完成签到,获得积分10
7秒前
9秒前
思源应助Nowind采纳,获得10
13秒前
YY完成签到,获得积分20
14秒前
FIB菜狗发布了新的文献求助10
16秒前
chen完成签到,获得积分10
17秒前
WuYiHHH完成签到,获得积分10
17秒前
19秒前
Ava应助WuYiHHH采纳,获得10
21秒前
桀桀桀发布了新的文献求助10
21秒前
24秒前
FIB菜狗完成签到,获得积分10
25秒前
发粪涂墙完成签到,获得积分10
26秒前
26秒前
我好想睡完成签到,获得积分10
26秒前
科目三应助文茵采纳,获得10
30秒前
桀桀桀发布了新的文献求助10
32秒前
灰原哀完成签到,获得积分10
34秒前
35秒前
35秒前
华仔应助科研通管家采纳,获得10
35秒前
Ava应助科研通管家采纳,获得10
35秒前
赘婿应助科研通管家采纳,获得10
35秒前
35秒前
思源应助科研通管家采纳,获得10
36秒前
科研通AI5应助科研通管家采纳,获得10
36秒前
我是老大应助科研通管家采纳,获得10
36秒前
YY发布了新的文献求助10
37秒前
脑洞疼应助SUE采纳,获得10
38秒前
38秒前
恒星的恒心完成签到 ,获得积分10
40秒前
IvyLee完成签到,获得积分10
41秒前
42秒前
00完成签到,获得积分10
42秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
Aktuelle Entwicklungen in der linguistischen Forschung 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989868
求助须知:如何正确求助?哪些是违规求助? 3531994
关于积分的说明 11255752
捐赠科研通 3270793
什么是DOI,文献DOI怎么找? 1805053
邀请新用户注册赠送积分活动 882215
科研通“疑难数据库(出版商)”最低求助积分说明 809208