高效液相色谱法
色谱法
化学
萃取(化学)
样品制备
出处
期刊:Amino Acids & Biotic Resources
日期:2004-01-01
被引量:1
摘要
To extract and purify type I collagen from pigskin, initial extraction and chromatography on semi -preparative HPLC were tested. The purity was determined on analysable HPLC. The physical and chemical characters of the sample were also determined. The test of acute toxicity, skin allergic were estimated. The target sample was found to be consistent with standard type I collagen. Type I collagen prepared by a combination of HPLC had high purity and favorable security .
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