化学
代谢组学
结直肠癌
机制(生物学)
蛋白质组学
癌症
计算生物学
色谱法
生物化学
内科学
医学
哲学
认识论
生物
基因
作者
Hengyuan Yu,Chen Yang,Jiayin Deng,Guoxin Cai,Weiliang Fu,Chengyu Shentu,Youdong Xu,Jie Liu,Yuan Zhou,Yingjie Luo,Yong Chen,Xuesong Liu,Yongjiang Wu,Tengfei Xu
标识
DOI:10.1016/j.jpba.2024.116379
摘要
Cannabis sativa L., with a rich history in Chinese folk medicine, includes hemp strains that offer substantial economic and medical benefits due to their non-addictive properties. Hemp has demonstrated various pharmaceutical activities, including anti-inflammatory, antioxidant, and anti-tumor effects. This study explores the potential of hemp oil extract (HOE) in treating colorectal cancer (CRC). Despite its promise, the specific anticancer mechanisms of HOE have not been well understood. To elucidate these mechanisms, we employed mass spectrometry-based metabolomics and proteomics to investigate the global effects of HOE on CRC cells. Additionally, bioinformatics approaches, including bulk RNA-seq and single-cell RNA-seq, were used to identify gene expression differences and cellular heterogeneity. The results were validated using flow cytometry, western blotting, and immunohistochemistry. Our findings reveal that HOE induces significant alterations in purine metabolism pathways, down-regulates c-MYC, and inhibits the expression of cell cycle-related proteins such as CCND1, CDK4, and CDK6, leading to cell cycle arrest in the G1 phase. This comprehensive analysis demonstrates that HOE effectively blocks the cell cycle in the G1 phase, thereby inhibiting colorectal cancer cell proliferation. These findings provide experimental evidence supporting the potential therapeutic use of hemp in medicine.
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