MPTP公司
线粒体通透性转换孔
化学
线粒体
生物物理学
细胞凋亡
线粒体膜转运蛋白
磁导率
程序性细胞死亡
细胞生物学
线粒体内膜
膜
生物化学
神经科学
生物
多巴胺能
多巴胺
作者
Minggang Tian,Baoli Dong,Zheming Zhang,Junling Yin,Weiying Lin
标识
DOI:10.1021/acs.analchem.1c04751
摘要
The opening of mitochondrial permeability transition pore (mPTP) plays a fundamental role in cell apoptosis regulation, ischemia-reperfusion injury, and neurodegenerative disorders. However, the molecular tools for detecting mPTP open in cellular native status have not been reported yet. Herein, we de novo designed a robust fluorescent probe mPTP-F to monitor mPTP opening in cellular native status for the first time. The membrane-permeable probe could accumulate into mitochondria and convert to a product poorly permeable to biomembranes, which was trapped in mitochondria to form near-infrared (NIR)-emissive aggregates. After mPTP opening, the product was released from mitochondria through the pore to form green-emissive monomers. Significantly, with mPTP-F, we discovered that formaldehyde, a signaling molecule, could induce mPTP opening. Therefore, the new probe could serve as a desirable molecular tool for the study of ischemia-reperfusion injury, cell apoptosis, and relative areas.
科研通智能强力驱动
Strongly Powered by AbleSci AI