右旋蔗糖酶
韦斯拉
右旋糖酐
明串珠菌
葡聚糖酶
化学
发酵
果糖
细菌
背景(考古学)
肠系膜明串珠菌
蔗糖
生物化学
水解
乳酸
乳酸菌
生物
古生物学
遗传学
作者
Norhane Besrour-Aouam,Imène Fhoula,Annel M. Hernández-Alcántara,Ma Luz Mohedano,Afef Najjari,Alicia Prieto,Patricia Rúas-Madiedo,Paloma López,Hadda‐Imene Ouzari
标识
DOI:10.1016/j.carbpol.2020.117254
摘要
High molecular weight dextrans improve the rheological properties of fermented products and have immunomodulatory and antiviral activity. We report on 5.84 × 107–2.61 × 108 Da dextrans produced by Leuconostoc lactis AV1n, Weissella cibaria AV2ou and Weissella confusa V30 and FS54 strains. Dextransucrases catalyze dextran synthesis by sucrose hydrolysis concomitant with fructose generation. The four bacteria have dextransucrases with molecular weight of about 160 kDa detected by zymograms. Each bacterium showed different interplay of dextran production and metabolic fluxes. All bacteria produced lactate, and AV2ou apart, synthesized mannitol from fructose. FS54 hydrolyzed dextran blue and the concentration of dextran produced by this bacterium decreased during the stationary phase. The AV1n binding to Caco-2 cells and polystyrene plates was higher under conditions for dextran synthesis. Thus, this is the first instance of a Weissella dextranase, associated with a dextransucrase ability, and of a positive influence of dextran on adhesion and aggregation properties of a bacterium.
科研通智能强力驱动
Strongly Powered by AbleSci AI