自噬
PI3K/AKT/mTOR通路
冬虫夏草
细胞凋亡
蛋白激酶B
细胞生物学
安普克
程序性细胞死亡
癌细胞
化学
细胞生长
癌症研究
磷酸化
生物
癌症
生物化学
蛋白激酶A
遗传学
食品科学
作者
Wucheng Qi,Xingtao Zhou,Junqiao Wang,Ke Zhang,Yujia Zhou,Shuping Chen,Shaoping Nie,Mingyong Xie
标识
DOI:10.1016/j.carbpol.2020.116113
摘要
Cordyceps sinensis is thought to have anti-cancer effects, but its mechanisms remain elusive. In this study, we aimed to investigate the anti-cancer effect of Cordyceps sinensis polysaccharide (CSP) on human colon cancer cell line (HCT116) and its mechanism. Results indicated that CSP significantly inhibited the proliferation of HCT116 cells, increased autophagy and apoptosis, while blocked autophagy flux and lysosome formation. Further experiments showed that CSP decreased the expression of PI3K and phosphorylation level of AKT and mTOR, increased the expression of AMPKa and phosphorylation level of ULK1. In addition, repression of CSP-induced autophagy by bafilomycin (autophagy inhibitor) enhanced apoptosis and cell death of HCT116 cells. Hence, our findings suggested that CSP inhibited the proliferation of HCT116 cells by inducing apoptosis and autophagy flux blockage, which might be achieved through PI3K-AKT-mTOR and AMPK-mTOR-ULK1 signaling. CSP may be a potential therapeutic agent for colon cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI