生物
逆转录酶
枯草芽孢杆菌
分子生物学
质粒
大肠杆菌
重组DNA
操纵子
核酸内切酶
蛋白酶
表达式向量
紫胶操纵子
病毒学
DNA
酶
生物化学
核糖核酸
基因
细菌
遗传学
作者
Stuart F. J. Le Grice,Verena Beuck,J Mous
出处
期刊:Gene
[Elsevier]
日期:1987-01-01
卷期号:55 (1): 95-103
被引量:58
标识
DOI:10.1016/0378-1119(87)90252-6
摘要
A 2.4-kb DNA fragment from the pol region of the human T-cell lymphotropic virus, encoding the protease, reverse transcriptase and a portion of the endonuclease N-terminus was stably introduced into a high-level Bacillus subtilis expression system under inducible control of the Escherichia coli lac regulatory elements. The major expression plasmid, pRTL11, contains a bacteriophage T5 promoter/lac operator element, which is controlled by lac repressor, supplied by the secondary plasmid, pBL1. Upon IPTG induction, a 90-kDa polyprotein is synthesised and subsequently proteolytically cleaved to reveal 64-kDa and 52-kDa polypeptides. Partial purification reveals that reverse transcriptase activity co-migrates with these two polypeptides.
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