已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Genomic Landscape and Tumor Mutational Burden Determination of Circulating Tumor DNA in Over 5,000 Chinese Patients with Lung Cancer

肺癌 循环肿瘤DNA 医学 癌症 DNA 癌症研究 生物 病理 遗传学
作者
Jie Shi,Zhiyu Wang,Junping Zhang,Yaping Xu,Xiao Xiao,Xiangming Quan,Ying Bai,Xia Yang,Zongjuan Ming,Xiaojin Guo,Huijing Feng,Xiaoling Yang,Xiaofei Zhuang,Fei Han,Kai Wang,Yonglei Shi,Yu Lei,Jun Bai,Shuanying Yang
出处
期刊:Clinical Cancer Research [American Association for Cancer Research]
卷期号:27 (22): 6184-6196 被引量:13
标识
DOI:10.1158/1078-0432.ccr-21-1537
摘要

Abstract Purpose: Having emerged as a noninvasive and clinically applicable approach for molecular determination of lung cancer, a genomic overview of circulating tumor DNA (ctDNA) of large-scale cohort may be helpful in novel biomarker development and therapeutic innovation. Experimental Design: Primary cohort encompasses 5,671 blood samples from 4,892 patients with lung cancer. Pair-wise tissue samples from 579 patients and additional 358 sample pairs were collected to evaluate the correlation between blood and tissue tumor mutational burden (TMB). Parallel sequencing with plasma/tissue and white blood cells was performed using a 1,021-gene panel. Results: Histologic subtyping was the most relevant to ctDNA detectability independent of other demographic characteristics, with small cell lung cancer showing the highest detectability, ctDNA abundance, and blood TMB (bTMB). Mutational landscape demonstrated significant differences, and integrated clonality analysis highlighted distinct driver-pattern and functional pathway interaction among various subtypes. The clonality and concurrent genes of EGFR mutations could predict the therapeutic efficacy of tyrosine kinase inhibitors (TKI), and RB1 mutations in non–small cell lung cancer characterized a subset with high bTMB, elevated ctDNA level, and potential small cell transformation. Most importantly, we developed an adjusted algorithm for bTMB in samples with extremely low ctDNA level and validated its correlation with tissue TMB in an independent cohort. Conclusions: ctDNA could serve as a promising alternative in genomic profiling for lung cancer. The novel identification of ctDNA clonality and adjusted bTMB might improve therapeutic and prognostic evaluation. This dataset was also a valuable resource for the development of new therapeutic targets and new genomically guided clinical trials.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
orixero应助景穆采纳,获得10
1秒前
4秒前
了一发布了新的文献求助10
4秒前
jyy应助刘坦苇采纳,获得10
5秒前
Singularity应助ZHAOYUN采纳,获得10
5秒前
7秒前
CC完成签到,获得积分10
8秒前
DDD发布了新的文献求助10
9秒前
Hello应助yuko采纳,获得10
10秒前
11秒前
焦立超发布了新的文献求助10
13秒前
小飞飞完成签到,获得积分10
13秒前
科目三应助默默洋葱采纳,获得30
15秒前
16秒前
同学甲完成签到 ,获得积分10
16秒前
16秒前
17秒前
淡然的舞仙完成签到 ,获得积分10
19秒前
19秒前
健壮元绿完成签到 ,获得积分20
20秒前
风清扬应助完美的从波采纳,获得30
23秒前
Ahiterin发布了新的文献求助80
23秒前
yuko发布了新的文献求助10
23秒前
MoonYC完成签到,获得积分10
25秒前
28秒前
28秒前
29秒前
菠萝完成签到 ,获得积分10
30秒前
蜀黍完成签到 ,获得积分10
30秒前
choo完成签到,获得积分10
30秒前
Tales完成签到,获得积分10
30秒前
默默千亦完成签到 ,获得积分10
30秒前
hzx发布了新的文献求助10
33秒前
欣喜惜筠完成签到,获得积分10
34秒前
35秒前
36秒前
ZHOUCHENG发布了新的文献求助10
41秒前
网上飞完成签到,获得积分10
42秒前
45秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976512
求助须知:如何正确求助?哪些是违规求助? 3520548
关于积分的说明 11203949
捐赠科研通 3257210
什么是DOI,文献DOI怎么找? 1798648
邀请新用户注册赠送积分活动 877835
科研通“疑难数据库(出版商)”最低求助积分说明 806555