Lanthanide-Doped Inorganic Nanoprobes for Luminescent Assays of Biomarkers

纳米材料 持续发光 光漂白 镧系元素 兴奋剂 光致发光 发光 纳米技术 发光测量 材料科学 化学 荧光 光电子学 光学 物理 离子 热释光 有机化学
作者
Luping Wang,Siyuan Han,Chenliang Li,Datao Tu,Xueyuan Chen
出处
期刊:Accounts of materials research [American Chemical Society]
卷期号:4 (2): 193-204 被引量:18
标识
DOI:10.1021/accountsmr.2c00233
摘要

ConspectusTremendous progress in nanomaterial and nanotechnology has been made in recent years, which greatly contributes to the development of inorganic nanoparticles (NPs) as luminescent probes in diverse biomedical applications. In particular, these luminescent nanoprobes are widely employed for sensitive assays of biomarkers like disease markers. Generally, the luminescent bioassay technologies mainly rely on conventional molecular probes such as lanthanide (Ln3+) chelates or organic dyes, which suffer from inferior photochemical stability, low photobleaching, potential long-term toxicity, or high background noise. In contrast, Ln3+-doped NPs possess distinct physicochemical properties including better photostability, lower toxicity, and superior optical properties like long photoluminescent (PL) lifetime, narrow emission band, and tunable spectral range from the ultraviolet to the second near-infrared (NIR-II), which make them extremely ideal as luminescent nanoprobes. As such, enormous research enthusiasm has been invested in this fascinating field of Ln3+-doped luminescent nanoprobes in recent years. Accordingly, background-free luminescent bioassays with high signal-to-noise have been achieved by employing Ln3+-doped NPs on the basis of their downshifting luminescence (DSL) with a long PL lifetime, NIR-II luminescence with long-wavelength emissions, or upconverting luminescence (UCL) upon NIR excitation. However, there are still key challenges for Ln3+-doped nanoprobes owing to their low brightness and quantum yield, which restrict their biomedical applications. During the past decade, we have explored efficient approaches for the synthesis and design of highly efficient Ln3+-doped nanoprobes toward ultrasensitive luminescent bioassay of disease markers.In this Account, we summarize our most recent endeavors toward the development of inorganic Ln3+-doped NPs as sensitive nanoprobes for luminescent bioassays. First, we overview the approaches of controlled synthesis and optical manipulation to obtain highly efficient Ln3+-doped NPs with desirable optical properties. Second, we survey the design of Ln3+-doped nanoprobes with outstanding water dispersibility and excellent biocompatibility through surface functional bioconjugation of NPs. By employing these nanoprobes, we propose and exemplify several background-free luminescent bioassay strategies in an effort to suppress the interference of background noise from scattered lights and autofluorescence from biological samples. Third, we highlight the ultrasensitive bioassay of disease markers such as the time-resolved luminescent bioassay, NIR-II luminescent bioassay, and UCL bioassay. Finally, the major challenges, promising emerging trends, and future perspectives on this attractive field are discussed. Through this Account, we aim to offer a series of effective approaches to luminescent bioassay with high sensitivity and excellent specificity based on Ln3+-doped nanoprobes, which may broaden the roadway for clinical bioassays and accelerate the exploration of novel nanoprobes in versatile biomedical applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
我是老大应助刻苦的书竹采纳,获得10
1秒前
智慧爷爷发布了新的文献求助10
1秒前
酷波er应助lw777采纳,获得10
1秒前
岁月如歌发布了新的文献求助10
3秒前
bin完成签到,获得积分10
5秒前
5秒前
很好完成签到,获得积分10
6秒前
小郝已读博完成签到 ,获得积分10
6秒前
6秒前
Self-made完成签到,获得积分10
6秒前
7秒前
8秒前
9秒前
岁月如歌完成签到,获得积分10
10秒前
11秒前
joey完成签到,获得积分10
12秒前
wei完成签到 ,获得积分10
13秒前
miaojuly发布了新的文献求助10
14秒前
14秒前
研友_LXjjOZ发布了新的文献求助150
14秒前
15秒前
16秒前
17秒前
坚定馒头完成签到,获得积分10
17秒前
18秒前
18秒前
星星应助科研通管家采纳,获得30
20秒前
y924758705完成签到,获得积分20
20秒前
打打应助科研通管家采纳,获得10
20秒前
坦率的匪应助科研通管家采纳,获得10
20秒前
烟花应助科研通管家采纳,获得10
20秒前
上官若男应助科研通管家采纳,获得10
20秒前
隐形曼青应助科研通管家采纳,获得10
20秒前
NexusExplorer应助科研通管家采纳,获得10
21秒前
21秒前
YamDaamCaa应助科研通管家采纳,获得30
21秒前
21秒前
21秒前
怡然乌应助科研通管家采纳,获得10
21秒前
21秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988868
求助须知:如何正确求助?哪些是违规求助? 3531255
关于积分的说明 11253071
捐赠科研通 3269858
什么是DOI,文献DOI怎么找? 1804822
邀请新用户注册赠送积分活动 881994
科研通“疑难数据库(出版商)”最低求助积分说明 809035