Are endothelial cell proliferation and mesenchymal transition as distinguishing characteristics of 3-week Sugen5416/hypoxia mice model?

Figure 1 (A) DEGs of endothelial cell proliferation (GO:0001935).Control group vs. SuHx group: three control groups, respectively, include 4935, 3429, and 3209 cells, and three SuHx groups, respectively, include 5127, 5805, and 2997 cells.(B) Expression level of ACTA2 in CDH5(+) and PECAM(+) cells.Three control groups, respectively, include 1875, 2020, and 1056 CDH5(+) and PECAM(+) cells and three IH groups, respectively, include 3084, 2075, and 2104 CDH5(+) and PECAM(+) cells.Significant difference tested by FindMarkers function of Seurat, Wilcox method was used for differential expression analysis, and both min.pctand logfc.thresholdwere set as 0.2.(C ) Expression proportion of ACTA2 in CDH5(+) and PECAM(+) cells.Control group vs. IH group: three control groups, respectively, include 478, 607, and 183 ACTA2(+) cells and three IH groups, respectively, include 819, 697, and 434 ACTA2(+) cells.Not significant test by unpaired t-test (P = 0.55).(D) Representative images of immunofluorescence double-stained of PECAM and ACTA2 in lung cryo-sections from mice under normoxia and three-week SuHx.