生物
视网膜
视网膜
神经科学
解剖
细胞生物学
生物化学
作者
Kenichi Toma,Mengya Zhao,Shaobo Zhang,Fei Wang,Hannah K. Graham,Jun Zou,Shweta Modgil,Wenhao H. Shang,Nicole Tsai,Zhishun Cai,Liping Liu,Guiying Hong,Arnold R. Kriegstein,Yang Hu,Jakob Körbelin,Ruobing Zhang,Yaping Joyce Liao,Tyson N. Kim,Xin Ye,Xin Duan
出处
期刊:Cell
[Elsevier]
日期:2024-05-01
卷期号:187 (11): 2767-2784.e23
被引量:2
标识
DOI:10.1016/j.cell.2024.04.010
摘要
The vasculature of the central nervous system is a 3D lattice composed of laminar vascular beds interconnected by penetrating vessels. The mechanisms controlling 3D lattice network formation remain largely unknown. Combining viral labeling, genetic marking, and single-cell profiling in the mouse retina, we discovered a perivascular neuronal subset, annotated as Fam19a4/Nts-positive retinal ganglion cells (Fam19a4/Nts-RGCs), directly contacting the vasculature with perisomatic endfeet. Developmental ablation of Fam19a4/Nts-RGCs led to disoriented growth of penetrating vessels near the ganglion cell layer (GCL), leading to a disorganized 3D vascular lattice. We identified enriched PIEZO2 expression in Fam19a4/Nts-RGCs. Piezo2 loss from all retinal neurons or Fam19a4/Nts-RGCs abolished the direct neurovascular contacts and phenocopied the Fam19a4/Nts-RGC ablation deficits. The defective vascular structure led to reduced capillary perfusion and sensitized the retina to ischemic insults. Furthermore, we uncovered a Piezo2-dependent perivascular granule cell subset for cerebellar vascular patterning, indicating neuronal Piezo2-dependent 3D vascular patterning in the brain.
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