间充质干细胞
生物
胰岛素
骨髓
细胞分化
流式细胞术
细胞生物学
细胞培养
干细胞
内科学
内分泌学
分子生物学
免疫学
医学
基因
生物化学
遗传学
作者
Layasadat Khorsandi,Fereshteh Nejaddehbashi,Akram Ahangarpour,Mahmoud Hashemitabar
出处
期刊:Tissue & Cell
[Elsevier]
日期:2015-02-01
卷期号:47 (1): 66-72
被引量:38
标识
DOI:10.1016/j.tice.2014.11.005
摘要
Fibrin glue (FG) is used in a variety of clinical applications and in the laboratory for localized and sustained release of factors potentially important for tissue engineering. The aim of this study was to evaluate FG scaffold effect on differentiation of insulin-producing cells (IPCs) from bone marrow-derived mesenchymal stem cells (BM-MSCs). In this experimental study BM-MSCs were cultured and the cells characterized by analysis of cell surface markers using flow cytometry. BM-MSCs were seeded in FG scaffold (3D culture) and then treated with induction media. After induction, the presence of IPCs was demonstrated using gene expression profiles for pancreatic cell differentiation markers (PDX-1, GLUT-2 and insulin) and insulin detection in cytoplasm. Release of insulin by these cells was confirmed by radioimmunoassay. Expression of the islet-associated genes PDX-1, GLUT-2 and Insulin genes in 3D cultured cells was markedly higher than the 2D cultured cells exposure differentiation media. Compared to 2D culture of BM-MSCs-derived IPCs, the insulin release from 3D BM-MSCs-derived IPCs showed a nearly 3 fold (p < 0.05) increase when exposed to a high glucose (25 mM) medium. Percentage of insulin positive cells in 3D experimental group showed an approximately 3.5-fold increase in compared to 2D experimental culture cells. The results of this study demonstrated that FG scaffold can enhance the differentiation of IPCs from rats BM-MSCs.
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