Structural insight into why S-linked glycosylation cannot adequately mimic the role of natural O-glycosylation

糖基化 聚糖 糖蛋白 化学 糖苷键 生物化学 N-连接糖基化 糖基转移酶
作者
Chao Chen,Bo Ma,Yefei Wang,Qiu Cui,Lishan Yao,Yaohao Li,Baoquan Chen,Yingang Feng,Zhongping Tan
出处
期刊:International Journal of Biological Macromolecules [Elsevier BV]
卷期号:253: 126649-126649 被引量:4
标识
DOI:10.1016/j.ijbiomac.2023.126649
摘要

There is an increasing interest in using S-glycosylation as a replacement for the more commonly occurring O-glycosylation, aiming to enhance the resistance of glycans against chemical hydrolysis and enzymatic degradation. However, previous studies have demonstrated that these two types of glycosylation exert distinct effects on protein properties and functions. In order to elucidate the structural basis behind the observed differences, we conducted a systematic and comparative analysis of 6 differently glycosylated forms of a model glycoprotein, CBM, using NMR spectroscopy and molecular dynamic simulations. Our findings revealed that the different stabilizing effects of S- and O-glycosylation could be attributed to altered hydrogen-bonding capability between the glycan and the polypeptide chain, and their diverse impacts on binding affinity could be elucidated by examining the interactions and motion dynamics of glycans in substrate-bound states. Overall, this study underscores the pivotal role of the glycosidic linkage in shaping the function of glycosylation and advises caution when switching glycosylation types in protein glycoengineering.
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