涂层
牛血清白蛋白
机制(生物学)
粘附
纳米技术
纳米材料
材料科学
细胞粘附
化学
生物物理学
色谱法
复合材料
生物
认识论
哲学
作者
Behafarid Ghalandari,Yingxin Yu,Farnaz Ghorbani,Antony R. Warden,Khalil Ahmad,Xiao Ming Sang,Shiyi Huang,Yu Zhang,Wenqiong Su,Adeleh Divsalar,Yiyang Li
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2021-01-01
卷期号:13 (47): 20098-20110
被引量:15
摘要
Protein coating is a strategy for modifying and improving the surface functional properties of nanomaterials. However, the underlying mechanism behind protein coating formation, which is essential for its practical applications, remains largely unknown. Herein, we investigate the fundamental molecular mechanism of protein coating formation. Polydopamine nanospheres (PDANS) coated with bovine serum albumin (BSA) are examined in this study due to their wide biomedical potential. Our results demonstrate that BSAs can flexibly bind to PDANS and maintain their structural dynamicity. Our findings unveil that regular structure formation arises from BSAs lateral interactions via electrostatic forces. Notably, the protein coating modified PDANS surface enhances cell adhesion and proliferation as well as osteogenic differentiation. Such an enhancement is attributed to complementary surface properties provided by the dynamic PDANS-BSA complex and regular structure caused by BSA-BSA interactions in protein coating formation. This study provides a fundamental understanding of the molecular mechanism of protein coating formation, which facilitates the further development of functional protein-coated nanomaterials and guides the bioengineering decision making for biomedical applications, especially in bone tissue engineering.
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