The in Vitro Antischistosomal Activity and Genotoxicity of the Active Ingredients of Allium sativum (allicin) and Curcuma longa (curcumin)

姜黄 姜黄素 曼氏血吸虫 遗传毒性 大蒜素 彗星试验 体外 毒理 化学 萝卜 生物 DNA断裂 传统医学 药理学 血吸虫病 毒性 DNA损伤 生物化学 植物 DNA 医学 细胞凋亡 蠕虫 程序性细胞死亡 免疫学 有机化学
作者
Hadeer Abd El-Hak Rashed,Ali H. Abu Almaaty,Maha F.M. Soliman,Nahla S. El-Shenawy
出处
期刊:Iranian Journal of Parasitology [Knowledge E]
被引量:1
标识
DOI:10.18502/ijpa.v16i1.5540
摘要

In this study, we assessed the in vitro antischistosomal activity of the active ingredients of Allium sativum (allicin) and Curcuma longa (curcumin) on Schistosoma mansoni.This study was conducted in Faculty of Science, Port said University, Egypt (2018). Adult worms were exposed to a range of concentrations of AL or CU, and worm survival was assessed 24 h post-exposure to calculate the lethal concentration of the compounds. Scanning electron microscopy was used to assess ultrastructural changes in the surface of AL- or CU- treated worms. The genotoxicities of AL and CU on S. mansoni were determined by DNA fragmentation analysis.We determined the concentrations of AL and CU required to kill 50% of S. mansoni (LC50 ). The LC50 of AL was 8.66 μL/mL, whereas 100% mortality of S. mansoni was achieved by AL at concentrations of 50 μL/mL. The LC50 of CU was 87.25 μL/mL, with the highest mortality of 91.3% seen after 24 h exposure to 100 μg/mL CU. Ultrastructural studies revealed that exposure to either AL or CU led to mild or severe surface damage to S. mansion, respectively. The degree of damage in the worms was sex-dependent. Interestingly, while CU exposure resulted in DNA fragmentation in S. mansoni worms, we observed no genotoxic effects of AL.Both AL and CU exhibit antischistosomal activity; the study provided evidence suggesting that these compounds act through distinct mechanisms. These promising results encourage further investigation into these compounds as potential antischistosomal agents, either alone or as complementary treatments to praziquantel.

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