Microbially induced clay weathering: Smectite-to-kaolinite transformation

Abstract Microbially induced formation and transformation of clay minerals are known to be ubiquitous in nature. This work investigated the smectite-to-kaolinite transformation by Bacillus mucilaginosus, a kind of silicate-weathering bacterium. Results showed that the microbe-smectite system doubled protein production compared with the abiotic controls and enhanced dissolved 1.6% of total Si and 0.9% of total Al from smectite after the 25 days experiment. The formation of kaolinite was verified through its distinguished d(001)-spacing of 0.710 nm revealed by synchrotron radiation X-ray diffraction (SR-XRD) and high-resolution transmission electron microscope (HR-TEM). HR-TEM analysis indicated some mixed layers of smectite and kaolinite appeared in the form of a super-lattice structure. Moreover, the compositional and morphological changes of the solids suggested the emergence of kaolinite was associated with the formation of amorphous SiO2 and fragmented clay particles with lower Si/Al ratio and exposed crystal edge. Based on the detection of –C=O species on the smectite surface and the decrease of pH from 8.5 to 6.5, we inferred the organic ligands secreted by Bacillus mucilaginosus complexed with cations, especially for Si, which stripped the tetrahedral sheets and promoted the kaolinization of smectite. To our knowledge, this is the first report of microbially induced smectite-to-kaolinite transformation under ambient conditions in a highly-efficient way. This work could shed light on a novel pathway of microbe-promoted weathering of smectite to kaolinite at the Earth surface conditions. Such a robust and efficient transformation from expansive smectite to non-expansive clays as kaolinite may be of great potential in enhancing oil recovery in reservoirs.