GelMA/TCP nanocomposite scaffold for vital pulp therapy

纳米复合材料 脚手架 材料科学 牙髓(牙) 生物医学工程 牙科 复合材料 医学
作者
Yuanyuan Han,Renan Dal‐Fabbro,Abdel H. Mahmoud,Maedeh Rahimnejad,Jinping Xu,Miguel Castilho,Waruna Lakmal Dissanayaka,Marco C. Bottino
出处
期刊:Acta Biomaterialia [Elsevier]
卷期号:173: 495-508 被引量:9
标识
DOI:10.1016/j.actbio.2023.11.005
摘要

Pulp capping is a necessary procedure for preserving the vitality and health of the dental pulp, playing a crucial role in preventing the need for root canal treatment or tooth extraction. Here, we developed an electrospun gelatin methacryloyl (GelMA) fibrous scaffold incorporating beta-tricalcium phosphate (TCP) particles for pulp capping. A comprehensive morphological, physical-chemical, and mechanical characterization of the engineered fibrous scaffolds was performed. In vitro bioactivity, cell compatibility, and odontogenic differentiation potential of the scaffolds in dental pulp stem cells (DPSCs) were also evaluated. A pre-clinical in vivo model was used to determine the therapeutic role of the GelMA/TCP scaffolds in promoting hard tissue formation. Morphological, chemical, and thermal analyses confirmed effective TCP incorporation in the GelMA nanofibers. The GelMA+20%TCP nanofibrous scaffold exhibited bead-free morphology and suitable mechanical and degradation properties. In vitro, GelMA+20%TCP scaffolds supported apatite-like formation, improved cell spreading, and increased deposition of mineralization nodules. Gene expression analysis revealed upregulation of ALPL, RUNX2, COL1A1, and DMP1 in the presence of TCP-laden scaffolds. In vivo, analyses showed mild inflammatory reaction upon scaffolds' contact while supporting mineralized tissue formation. Although the levels of Nestin and DMP1 proteins did not exceed those associated with the clinical reference treatment (i.e., mineral trioxide aggregate), the GelMA+20%TCP scaffold exhibited comparable levels, thus suggesting the emergence of differentiated odontoblast-like cells capable of dentin matrix secretion. Our innovative GelMA/TCP scaffold represents a simplified and efficient alternative to conventional pulp-capping biomaterials. Vital pulp therapy (VPT) aims to preserve dental pulp vitality and avoid root canal treatment. Biomaterials that bolster mineralized tissue regeneration with ease of use are still lacking. We successfully engineered gelatin methacryloyl (GelMA) electrospun scaffolds incorporated with beta-tricalcium phosphate (TCP) for VPT. Notably, electrospun GelMA-based scaffolds containing 20% (w/v) of TCP exhibited favorable mechanical properties and degradation, cytocompatibility, and mineralization potential indicated by apatite-like structures in vitro and mineralized tissue deposition in vivo, although not surpassing those associated with the standard of care. Collectively, our innovative GelMA/TCP scaffold represents a simplified alternative to conventional pulp capping materials such as MTA and Biodentine™ since it is a ready-to-use biomaterial, requires no setting time, and is therapeutically effective.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
科研通AI2S应助jinshijie采纳,获得10
1秒前
4秒前
4秒前
4秒前
今后应助爱听歌土豆采纳,获得10
5秒前
深情安青应助科研通管家采纳,获得10
5秒前
嗯哼应助科研通管家采纳,获得20
5秒前
科研通AI2S应助科研通管家采纳,获得10
5秒前
爆米花应助科研通管家采纳,获得10
5秒前
嗯哼应助科研通管家采纳,获得20
5秒前
科研通AI2S应助科研通管家采纳,获得10
5秒前
5秒前
5秒前
5秒前
科研通AI2S应助草木采纳,获得10
5秒前
6秒前
6秒前
毛毛虫完成签到,获得积分10
6秒前
高君奇发布了新的文献求助10
7秒前
9秒前
zzyfsh发布了新的文献求助10
9秒前
ZJJZ发布了新的文献求助30
10秒前
快乐的小蜗牛完成签到,获得积分10
10秒前
小郭不洗锅完成签到,获得积分20
10秒前
11秒前
汉堡包应助动听的夏真采纳,获得10
14秒前
14秒前
在水一方应助炙热的夜雪采纳,获得10
15秒前
15秒前
15秒前
淡淡的小蘑菇完成签到 ,获得积分10
16秒前
朴实香露发布了新的文献求助10
16秒前
哆啦猫发布了新的文献求助10
17秒前
852应助慕迎蕾采纳,获得10
17秒前
17秒前
18秒前
20秒前
云岫完成签到,获得积分10
20秒前
大黄发布了新的文献求助30
20秒前
ZLY完成签到,获得积分10
21秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Diamonds: Properties, Synthesis and Applications 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3098628
求助须知:如何正确求助?哪些是违规求助? 2750398
关于积分的说明 7608117
捐赠科研通 2402221
什么是DOI,文献DOI怎么找? 1274557
科研通“疑难数据库(出版商)”最低求助积分说明 616124
版权声明 599033