[Research progress of Lgr5-positive stem cells in the formation of organoid in 3D culture].

Stem cell is critical to regeneration of tissue or organ of human. How to promote repair or regeneration in the tissues/organ using its pluripotency is always an important issue. Lgr5-possitive cell is one type of the stem cell-like cells capable of pluripotent differentiation in various tissues/organs of both humans and mice. Current study showed that single or small amount Lgr5-possitive stem cells can grow and form a plurality of organs in 3D culture system, and some organs can present similar biological and physiological properties with the progenitor they were derived. These studies provided new insight into future orientation, for example, Lgr5-possitive inner ear cells were confirmed as inner ear pluripotent cells population, the experiences obtained from organoid studies of Lgr5-possitive cells have certainly showed potential in the future study of inner ear stem cells. This review will focus on the recent progress associated with Lgr 5-positive stem cells forming organoids in the 3D culture.干细胞是人体中组织器官再生的基石，如何充分利用其多能性促进机体细胞、组织和器官的修复或再生是生物医学研究中长久以来的热点和重点。Lgr5阳性细胞是机体中具有多能性分化能力的干细胞样细胞之一，存在于小鼠或人类的多种器官或组织中。目前研究表明，单个或少量的Lgr5阳性干细胞可以在3D培养体系中生长形成多种类器官(organoid)，这些类器官的某些生物学及生理学特性与其来源的器官类似或一致。这些实验拓宽了研究者的视野，同时也开辟了一种新的干细胞研究方向。对于内耳细胞再生研究来说，Lgr5阳性内耳干细胞正是近年来刚被证实的内耳多能性细胞群。如何从来源于Lgr5阳性干细胞的类器官研究中获得灵感或经验，对于内耳干细胞的研究具有前瞻性指导意义。本文将对Lgr5阳性干细胞在3D培养体系中形成类器官的相关研究进行综述，以期为类内耳器官的形成与应用提供新思路。.