Bioactive chemical constituents from Rhodiola crenulata and effect on hypoxia inducible factor-1α expression

OBJECTIVE To study the bioactive chemical constituents of Rhodiola crenulata and the effect on hypoxia inducible factor-1α(HIF-1α)expression.METHODS The chemical constituents were isolated and purified using silica gel column chromatography.Their structures were determined on the basis of NMR and mass spectra.The effect of chemical constituents on the expression of HIF-1α mRNA were measured by RT-PCR.RESULTS Five Compounds were isolated from the active fraction of Rhodiola crenulata.These chemical structures were identified as rhodiolin(Ⅰ),rhodiolatuntoside(Ⅱ),gallic acid(Ⅲ),tyrosol(Ⅳ)and salidroside(Ⅴ).compounds Ⅳ and Ⅴ can up-regulate the expression of HIF-1α mRNA(P0.01).CONCLUSION The configuration of compound Ⅰ was confirmed by 2D-NMR and MS methods.Compounds Ⅳ and Ⅴ can up-regulate expression of HIF-1α mRNA.