古细菌
细菌
基因组
染色体
计算生物学
生物
细菌圆形染色体
基因
分辨率(逻辑)
遗传学
计算机科学
程序设计语言
作者
Charlotte Cockram,Agnès Thierry,Romain Koszul
出处
期刊:STAR protocols
[Elsevier]
日期:2021-05-05
卷期号:2 (2): 100512-100512
被引量:10
标识
DOI:10.1016/j.xpro.2021.100512
摘要
Chromosome conformation capture (Hi-C) has become a routine method for probing the 3D organization of genomes. However, when applied to bacteria and archaea, current protocols are expensive and limited in their resolution. By dissecting the different steps of published eukaryotic and prokaryotic Hi-C protocols, we have developed a cost- and time-effective approach to generate high-resolution (down to 500 bp – 1 kb) contact matrices of both bacteria and archaea genomes. For complete details on the use and execution of this protocol, please refer to Cockram et al. (2020).
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