清脆的
基因组编辑
核糖核蛋白
Cas9
计算生物学
生物
计算机科学
遗传学
基因
核糖核酸
作者
Hannah Karp,Madeline Zoltek,Kevin Wasko,Angel Menendez Vazquez,Jinna Brim,Wayne Ngo,Alanna Schepartz,Jennifer A. Doudna
标识
DOI：10.1101/2024.10.18.619117
摘要
Effective genome editing requires a sufficient dose of CRISPR-Cas9 ribonucleoproteins (RNPs) to enter the target cell while minimizing immune responses, off-target editing and cytotoxicity. Clinical use of Cas9 RNPs currently entails electroporation into cells
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