顺铂
自噬
癌症研究
细胞凋亡
A549电池
基因敲除
化学
DNA损伤
肺癌
细胞生物学
生物
医学
化疗
DNA
肿瘤科
生物化学
内科学
作者
Beinuo Wang,Ruijie Zhang,Wei Wang,Hang Qian,Di Wu,Binfeng He,Hu Liao
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2023-04-03
卷期号:24 (5): 2063-2074
被引量:1
标识
DOI:10.1021/acs.biomac.2c01493
摘要
Overcoming cisplatin-based drug resistance in lung cancer remains an enormous challenge in clinical tumor therapy worldwide. Recent studies have reported that some Rab GTPases are involved in multiple aspects of tumor progression, including invasion, migration, metabolism, autophagy, exosome secretion, and drug resistance. In particular, Rab26 is essential to vital processes such as vesicle-mediated secretion, cell growth, apoptosis, and autophagy. In this study, we developed a nanosystem based on programmed DNA self-assembly of Rab26 siRNA-loaded nanoparticles (siRNP). We demonstrated that siRNP could be effectively transfected into cisplatin-resistant A549 (A549/DDP) cells. These siRab26-carrying nanoparticles induced apoptosis and inhibited the disruption of autophagy. The combination therapy of siRab26 knockdown with cisplatin could improve the antitumor therapy compared with a single one in vitro. In nude mice, siRNP enhanced the chemosensitivity of cisplatin-resistant cells and inhibited tumor xenograft development. These outcomes suggest that siRNP is an effective platform for lung cancer therapy in cases exhibiting drug resistance.
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