Synergistic Antimicrobial Activity of Silver Nanoparticles with an Emergent Class of Azoimidazoles

抗菌剂 哈卡特 银纳米粒子 白色念珠菌 化学 聚乙烯吡咯烷酮 动态光散射 最小抑制浓度 细胞毒性 细菌 金黄色葡萄球菌 大肠杆菌 微生物学 核化学 纳米颗粒 纳米技术 生物化学 材料科学 生物 有机化学 体外 遗传学 基因
作者
Ana Isabel Ribeiro,Bárbara Vieira,Daniela Dantas,Bárbara Silva,Eugénia Pinto,Fátima Cerqueira,Renata Silva,Fernando Remião,Jorge Padrão,Alice Dias,Andréa Zille
出处
期刊:Pharmaceutics [MDPI AG]
卷期号:15 (3): 926-926 被引量:9
标识
DOI:10.3390/pharmaceutics15030926
摘要

The combination of two or more agents capable of acting in synergy has been reported as a valuable tool to fight against pathogens. Silver nanoparticles (AgNPs) present a strong antimicrobial action, although their cytotoxicity for healthy cells at active concentrations is a major concern. Azoimidazole moieties exhibit interesting bioactivities, including antimicrobial activity. In this work, a class of recently described azoimidazoles with strong antifungal activity was conjugated with citrate or polyvinylpyrrolidone-stabilized AgNPs. Proton nuclear magnetic resonance was used to confirm the purity of the compounds before further tests and atomic absorption spectroscopy to verify the concentration of silver in the prepared dispersions. Other analytical techniques elucidate the morphology and stability of AgNPs and corresponding conjugates, namely ultraviolet-visible spectrophotometry, scanning transmission electron microscopy and dynamic light scattering analysis. The synergistic antimicrobial activity of the conjugates was assessed through a checkerboard assay against yeasts (Candida albicans and Candida krusei) and bacteria (Staphylococcus aureus and Escherichia coli). The conjugates showed improved antimicrobial activity against all microorganisms, in particular towards bacteria, with concentrations below their individual minimal inhibitory concentration (MIC). Furthermore, some combinations were found to be non-cytotoxic towards human HaCaT cells.
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