纳米孔
低聚物
DNA
单体
信号(编程语言)
材料科学
聚合
纳米技术
微流控
分辨率(逻辑)
组合化学
生物系统
化学
计算机科学
聚合物
生物
高分子化学
生物化学
复合材料
程序设计语言
人工智能
作者
Yesheng Wang,Zhentong Zhu,Chunmiao Yu,Ruiping Wu,Jinbo Zhu,Bingling Li
出处
期刊:Small
[Wiley]
日期:2022-11-27
卷期号:19 (4)
被引量:10
标识
DOI:10.1002/smll.202206283
摘要
While the solid-state nanopore shows increasing potential during sensitive and label-free single molecular analysis, target concentration and signal amplification method is in urgent need. In this article, a solution via designing a model nucleic acid circuit reaction that can produce "Y" shape-structure three-way DNA oligomers with controllable size and polymerization degree is proposed. Such a so-called lego-like three-way catalytic hairpin assembly (LK-3W-CHA) can provide both concentration amplification (via CHA circuit) and programmable size control (via lego-like building mode) to enhance spatiotemporal resolution in single molecular sensing of solid-state nanopore. Oligomers containing 1-4 DNA three-way junctions (Y monomers, Y1-Y4) are designed in proof-of-concept experiments and applications. When the oligomers are applied to direct translocation measurements, Y2-Y4 can significantly increase the signal resolution and stability than that of Y1. Meanwhile, Y1 to Y4 can be used as the tags on the long DNA carrier to provide very legible secondary signals for specific identification, multiple assays, and information storage. Compared with other possible tags, Y1-Y4 provides higher signal density and amplitude, and quasi-linear "inner reference" for each other, which may provide more systematic, reliable, and controllable experimental results.
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