Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens

Conventional transmission electron microscopy is an essential tool to understand the structure–function relationships and play a vital role in biological research. Mitochondria-associated membranes are linked with cancer processes in a fundamental manner. A conventional transmission electron microscopy method for preparing specimens in clinical and research settings for the study-analysis of the mitochondria-associated membranes in human tumors is presented. The sample processing includes chemical fixation by immersion, dehydration, embedding, polymerization, sectioning, and staining.