硫酸酯酶
纳米探针
甾体硫酸酯酶
化学
酶
癌症研究
生物物理学
生物化学
纳米技术
材料科学
生物
纳米颗粒
激素
类固醇
作者
Yanqi Xu,Mengyuan Cui,Wancun Zhang,Tianguang Liu,Xiangyu Ren,Yueqing Gu,Chongzhao Ran,Jing Yang,Peng Wang
标识
DOI:10.1016/j.cej.2021.132514
摘要
Tracking sulfatase in the living organism is beneficial for the diagnosis of sulfatase-overexpressed cancer and understanding the pathological activities of sulfatase. A nanoprobe (DQM-SULF) with AIE signal amplification for imaging of sulfatase activity was rationally developed. The probe could be self-assembled into nanoparticles in the aqueous solution due to the hydrophobic AIE core and hydrophilic sulfatase-recognition unit. DQM-SULF could be activated by the catalytic domain of sulfatase to afford the AIEgen DQM-OH, which can emit the aggregation-induced fluorescence and further bind to the hydrophobic site of sulfatase resulted in a stronger AIE signal. The nanoprobe exhibited a fast response and high sensitivity to sulfatase. Moreover, it was successfully employed in detecting the endogenous overexpressed sulfatase in tumor cells and tumor tissue. Importantly, the sulfatase activity in a mouse metastatic lung tumor model was imaged using DQM-SULF by pulmonary administration, indicating DQM-SULF has the potential to detect cancer cells as the inhalation contrast medium.
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