Involvement of Rictor/mTORC2/Akt/GLUT4 pathway in the regulation of energy metabolism in the gastric smooth muscle of diabetic rats

过剩4 mTORC2型 PI3K/AKT/mTOR通路 蛋白激酶B 内分泌学 内科学 葡萄糖转运蛋白 信号转导 生物 mTORC1型 胰岛素 细胞生物学 医学
作者
Sun Yan,Yurong Zheng,Zheng Jin,Mohan Zhang,Xiang‐Shu Cui
出处
期刊:Acta Biochimica Polonica [Polskie Towarzystwo Biochemiczne (Polish Biochemical Society)]
被引量:2
标识
DOI:10.18388/abp.2020_5652
摘要

Diabetes mellitus can be accompanied by a variety of complications. The purpose of the present study was to characterize the Rictor/mTOR complex 2 (mTORC2)/Akt/glucose transporter 4 (GLUT4) pathway and its effects on energy metabolism in the gastric smooth muscle of diabetic rats. Diabetes was induced in rats using streptozotocin and their phenotype was compared with untreated rats. The relationship between gastric motility and energy metabolism was analyzed by comparing the contraction and ATP metabolism of muscle strips. Western blotting was used to detect the expression of key proteins in the pathway. The diabetic rats demonstrated less frequent and less powerful gastric smooth muscle contractions. The concentrations of ADP, AMP, and ATP, and the energy charge in gastric smooth muscle changed in different periods of diabetes, and these changes were consistent with changes in mechanistic target of rapamycin (mTOR) protein content. The expression of the key intermediates in signal transduction in the Rictor/mTORC2/Akt/GLUT4 pathway also underwent significant changes. Rictor protein expression increased during the development of diabetes, but the activation of mTORC2 did not increase with the increase in Rictor expression. GLUT4 translocation is regulated by Akt and its expression change during the development of diabetes. These findings suggest that altered energy metabolism is present in gastric smooth muscle that is associated with changes in the Rictor/mTORC2/Akt/GLUT4 pathway. Rictor/mTORC2/Akt/GLUT4 pathway may be involved in the regulation of energy metabolism in the gastric smooth muscle of diabetic rats and the development of diabetic gastroparesis.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
Huang完成签到 ,获得积分0
1秒前
吧啦吧啦发布了新的文献求助10
1秒前
嘀嘀哒哒发布了新的文献求助30
1秒前
2秒前
2秒前
Mingda发布了新的文献求助10
3秒前
aikanwenxian发布了新的文献求助10
3秒前
和谐静竹发布了新的文献求助10
3秒前
xiao完成签到,获得积分10
3秒前
zho发布了新的文献求助10
4秒前
niuniu完成签到,获得积分20
4秒前
干净的怜珊完成签到,获得积分10
4秒前
6秒前
7秒前
大个应助诸以晴采纳,获得10
7秒前
TianY天翊发布了新的文献求助10
7秒前
夏雨的天发布了新的文献求助20
8秒前
dyyisash完成签到 ,获得积分10
9秒前
饼哥完成签到,获得积分10
9秒前
劲秉应助嘀嘀哒哒采纳,获得10
9秒前
10秒前
小蘑菇应助冷酷豌豆采纳,获得10
10秒前
Mike发布了新的文献求助10
12秒前
DS应助哒哒哒采纳,获得100
12秒前
12秒前
大模型应助子车浩宇采纳,获得10
13秒前
13秒前
山野完成签到,获得积分10
13秒前
14秒前
画画的baby完成签到 ,获得积分10
14秒前
LT完成签到,获得积分10
14秒前
15秒前
15秒前
17秒前
111发布了新的文献求助10
17秒前
zz完成签到,获得积分10
17秒前
18秒前
芽芽乐发布了新的文献求助10
18秒前
peanut完成签到,获得积分10
18秒前
高分求助中
Continuum Thermodynamics and Material Modelling 4000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
El viaje de una vida: Memorias de María Lecea 800
Novel synthetic routes for multiple bond formation between Si, Ge, and Sn and the d- and p-block elements 700
Neuromuscular and Electrodiagnostic Medicine Board Review 700
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3515049
求助须知:如何正确求助?哪些是违规求助? 3097391
关于积分的说明 9235300
捐赠科研通 2792358
什么是DOI,文献DOI怎么找? 1532422
邀请新用户注册赠送积分活动 712063
科研通“疑难数据库(出版商)”最低求助积分说明 707107