生物
配子发生
作文(语言)
酶
功能(生物学)
代谢途径
遗传学
生物化学
细胞生物学
基因
语言学
哲学
胚胎发生
作者
Jannik Hugener,Jianlin Xu,Rahel Wettstein,Lydia Ioannidi,Daniel Velikov,Florian Wollweber,Adrian Henggeler,Joao Matos,Martin Pilhofer
出处
期刊:Cell
[Elsevier]
日期:2024-06-01
卷期号:187 (13): 3303-3318.e18
被引量:1
标识
DOI:10.1016/j.cell.2024.04.026
摘要
Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification" (FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4ALDH2 and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1ACSS2. Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts.
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