唾液酸转移酶
乳糖神经酰胺
神经节苷脂
唾液酸
生物化学
化学
酶
ATP合酶
糖基转移酶
生物合成
作者
Kei-ichiro Inamori,Takahiro Nitta,Fumi Shishido,Soichiro Watanabe,Isao Ohno,Jin-ichi Inokuchi
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 101-110
被引量:1
标识
DOI:10.1007/978-1-0716-2910-9_9
摘要
GM3 synthase (GM3S) is a sialyltransferase that transfers sialic acid from CMP-sialic acid to lactosylceramide. This reaction results in formation of ganglioside GM3 and is essential for biosynthesis of its downstream derivatives, which include a- and b-series gangliosides. Here, we describe a method for GM3S enzymatic assay using fluorescence-labeled alkyl lactoside as acceptor substrate, followed by HPLC for separation of enzymatic product. The method allows quantitative assay of GM3S sialyltransferase activity in cultured cells and mouse brain tissues.
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